top of page

 Link to Pubmed and Google Scholar


Ália dos Santos, Daniel E. Rollins, Yukti Hari-Gupta, Hannah C. W. Reed, Mingxue Du, Sabrina Yong Zi Ru, Kseniia Pidlisna, Ane Stranger, Faeeza Lorgat, Ian Brown, Kevin Howland, Jesse Aaron, Lin Wang, Peter J. I. Ellis, Teng-Leong Chew, Marisa Martin-Fernandez, Alice L. B. Pyne, Christopher P. Toseland

Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription“. Nature Communications 2023 14 2855

Shahid-Fuente, I.  & Toseland, C.P. "Myosin in chromosome organisation and gene expression" Biochemical Society Transactions 2023 51 1023-1034.

Gonzalez-Novo, R., Zamora Carreras, H., De Lope-Planelles, A., Lopez-Menendez, H., Roda-Navarro, P., Monroy, F., Wang, L., Toseland, C.P. & Redondo-Munoz, J. “3D environment modulates persistent lamin distribution and the biochemical signature of the nucleus”. BioRxiv 2023 


dos Santos, A., Fili, N., Hari-Gupta, Y., Gough, R.E., Wang, L., Martin-Fernandez, M., Arron, J., Waite, E., Chew, T.L. & Toseland, C.P. “Binding partners regulate unfolding of myosin VI to activate the molecular motor”. Biochemical Journal 2022 479 1409-1428.

dos Santos, Á. Gough, R.E., Wang, L. & Toseland, C.P. "Measuring Nuclear Organization of Proteins with STORM Imaging and Cluster Analysis". Chromosome Architecture. Methods in Molecular Biology, 2022, 293-309. 

dos Santos, Á. Rehfeldt, F. & Toseland, C.P. "Measuring Nuclear Mechanics with Atomic Force Microscopy". Chromosome Architecture. Methods in Molecular Biology, 2022, 171-181. 

Hari-Gupta, Y., Fili, N., dos Santos, A., Cook, A.W., Gough, R.E., Reed, H.C.W., Wang, L., Arron, J., Venit, T., Waite, E., Grosse-Berkenbusch, A., Gebhardt, J.C.M., Percipalle, P., Chew, T.L.,  Martin-Fernandez, M. & Toseland, C.P. “Nuclear myosin VI regulates the spatial organization of mammalian transcription initiation“. Nature Communications 2022 13 1346

dos Santos, Á. & Toseland, C.P. "Magnetic Tweezers in a microplate format". J. Vis. Exp. 2022 (180), e62994 


dos Santos, A. & Toseland, C.P. "Regulation of Nuclear Mechanics and the Impact on DNA Damage" Int. J. Molecular Sciences 2021 22 3178.

Emmanouilidis, I., Fili, N., Cook, A., Hair-Gupta, Y., dos Santos, A., Wang, L., Martin-Fernandez, M., Ellis, P.E.J. & Toseland, C.P. "A targeted and tuneable DNA damage tool using CRISPR/Cas9" Biomolecules 2021 11 288.

dos Santos, A., Fili, N., Pearson, D., Hari-Gupta, Y. & Toseland, C.P. “High throughput mechanobiology: Force modulation of ensemble biochemical and cell-based assays”. Biophysical Journal 2021 120 631-641.

dos Santos, A., Cook., A.W., Gough, R.E., Shilling, M., Aleida Olszok, N., Brown, I., Wang, L., Arron, J., Martin-Fernandez, M.L., Rehfeldt, F. & Toseland, C.P. "DNA damage alters nuclear mechanics through chromatin reorganisation​". Nucleic Acids Research 2021 49 340-353.


Cook, A.W. & Toseland, C.P. "The roles of nuclear Myosins in the DNA damage response. J Biochem. 2020:

Cook, A.W., Gough, R.E. & Toseland, C.P. "Nuclear Myosins - roles for molecular transporters and anchors". J Cell Science 2020 133:

Fili, N., Hari-Gupta, Y., Aston, B., dos Santos, A., Gough, R.E., Alamad, B., Wang, L., Martin-Fernandez, M. & Toseland, C.P. “Competition between two high- and low-affinity protein-binding sites in myosin VI controls its cellular function”. J Biol. Chem. 2020 295:337 

Fili, N. & Toseland, C.P. “Unconventional Myosins: How Regulation Meets Function”. Int. J. Molecular Sciences. 2020 21:67

Grosse-Berkenbusch, A., Hettich, J., Kuhn, T., Fili, N., Cook, A.W., Hari-Gupta, Y., Palmer, A., Streit, L., Ellis, P.J.I., Toseland, C.P. & Gebhardt, J.C.M. “Myosin VI moves on nuclear actin filaments and supports long-range chromatin rearrangements”. Biorxiv 2020


Acton, S.E., Bell, A.J.D., Toseland, C.P. and Twelvetress, A. "A survey of new PIs in the UK" eLife 2019 8:e46827


Cook, A.,  Hari-Gupta, Y. & Toseland, C.P.  “Application of the SSB biosensor to study in vitro transcription”. Biochem. Biophys. Res. Commun. 2018 496:820.


Toseland, C.P.  “Function of Nuclear Myosin Motors”. Impact. 2017 10:49-51.

Fili, N.,  Hari-Gupta, Y., Dos Santos, A.,  Cook, A., Poland, S., Ameer-Beg, SM., Parsons, M. & Toseland, C.P.  “NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription.”. Nature Communications. 2017 8:1871.

Lherbette, M.,  Dos Santos, A., Hari-Gupta, Y., Fili, N., Toseland, C.P. & Schaap, I.A.T. “Atomic Force Microscopy micro-rheology reveals large structural inhomogeneities in single cell-nuclei”. Scientific Reports. 2017 7:8116.


Yao, M.,  Goult, B.T., Klapholz, B., Toseland, C.P., Guo, Y., Con, P., Cheetz, M.P. & Yan, J. “The Mechanical Response of Talin”. Nature Communications. 2016 7:11966.


Wilhelm, L.,  Bürmann, F., Minnen, A., Shin, H.C., Toseland, C.P., Oh, B.H. & Gruber, S. “SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis”. eLife 2015

Kang, H.A.,  Shin, H.C., Kalantzi, A.S., Toseland, C.P., Kim, H.M., Gruber, S., Dal Peraro, M. & Oh, B.H.“Crystral Structure of Hop2-Mnd1 and mechanistic insights into its role in meiotic recombination”. Nucleic Acid Res. 2015 43:3841 

Soh, Y.M., Bürmann, F., Shin, H.C., Oda, T., Jin, K.S., Toseland, C.P., Kim, C., Lee, H., Kim, S. J., Kong, M.S., Durand-Diebold, M.L., Kim, Y., Kim, H.M., Lee, N.K., Sato, M., Oh, B.H. & Gruber, S. “Molecular basis for SMC rod formation and its dissolution upon DNA binding”. Mol. Cell. 2015 57:290-303. 


BOOK: Fluorescent Methods for Molecular Motors. Eds. Toseland, C.P. & Fili, N. 

Toseland, C.P. “Fluorescence to study the ATPase mechanism of motor proteins”. Experientia supplemetum. 2014 105:67-86

Toseland, C.P. & Geeves, M. “Rapid reaction kinetic techniques”. Experientia supplemetum. 2014 105:49-65.

Fili, N. & Toseland, C.P. “Fluorescence and Labelling: How to choose and what to do” Experientia supplementum. 2014 105:1-24.


Toseland, C.P. & Webb, M.R. “ATPase mechanism of the 5′-3′ DNA helicase, RecD2: evidence for a pre-hydrolysis conformation change.” J. Biol. Chem. 2013 288:25183.

Toseland, C.P. “Fluorescent labelling and modification of Proteins”. J. Chem. Biol. 2013 6:85-95.

Chisty, L., Toseland, C.P., Fili, N. Mashanov, G.I., Dillingham, M.S., Molloy, J.E. & Webb, M.R. “Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD.” Nucleic Acids Research 2013 41:5010-5023. (Joint First Authors) ***NAR Featured Article

Webb, M.R. & Toseland, C.P.  “Helicases”. In Encyclopedia of Biophysics. 2013 950-958.


Batters, C., Woodall, K.A., Toseland, C.P., Hundschell, C. & Veigel, C.  “Cloning, expression and characterisation of a novel molecular motor, Leishmania myosin-XXI”. J. Biol. Chem. 2012 287:27556.

Toseland, C.P., Powell, B. & Webb, M.R.  “ATPase and DNA unwinding kinetics of RecG helicase”. PLoS One 2012 7:e38270


Toseland, C.P. & Webb, M.R. “Fluorescence nucleoside triphosphates for single molecule enzymology”. Methods in Molecular Biology 2011 778:161.

Fili, N., Toseland, C.P., Dillingham, M.S., Webb, M.R. and Molloy, J.E. “A single molecule approach to visualize DNA unwinding”. Methods in Molecular Biology 2011 778:193.


Fili, N., Mashanov, G.I., Toseland, C.P., Batters, C., Wallace, M.I., Yeeles, J.T.P., Dillingham, M.S., Webb, M.R. and Molloy, J.E. “Visualizing helicases unwinding DNA at the single molecule level”. Nucleic Acids Research 2010 38:4448.

Toseland, C.P. & Webb, M.R.  “Fluorescence tools to measure helicase activity in real-time”. 2010 Methods 51:259.


Toseland, C.P., Slatter, A.F., Martinez-Senac, M.M. & Webb, M.R  “The ATPase cycle of PcrA Helicase and its coupling to translocation on DNA.” J. Mol. Biol. 2009 392:1020-1032.


Empirical, AI, and QSAR Approaches to Peptide-MHC Binding Prediction. Hattotuwagama, C.K., Guan, P., Davies, M., Taylor, D.J., Walshe, V., Hemsley, S.L., Toseland, C., Doytchinova, I.A., Borrow, P. and Flower, D.R.  In ‘In silico Immunology’ Ed. Flower, D.R. & Timmis, J. 2007

Davies M.N., Guan P., Blythe M.J., Salomon J., Toseland C.P., Hattotuwagama C., Walshe V., Doytchinova I.A., & Flower D.R.  Using databases and data-mining in Vaccinology.  Expert opinion on Drug Discovery 2007 2:19-35.


Taylor P.D., Toseland C.P., Attwood T.K., Flower D.R.  LIPPred: A web server for accurate prediction of lipoprotein signal sequences and cleavage sites.  Bioinformation 2006 1, 176-179.

Taylor P.D., Toseland C.P., Attwood T.K., Flower D.R. TATPred: a Bayesian method for the identification of twin arginine translocation pathway signal sequences.  Bioinformation 2006 1, 184-187.

Taylor P.D., Toseland C.P., Attwood T.K., Flower D.R.  A Predictor of Membrane Class:  Discriminating α-helical and β-barrel membrane proteins from non-membranous proteins.  Bioinformation 2006 1, 208-213.

Taylor P.D., Toseland C.P., Attwood T.K., Flower D.R.  Beta Barrel Transmembrane Proteins: Enhanced prediction using a Bayesian approach.  Bioinformation 2006 1, 231-233.

Taylor P.D., Toseland C.P., Attwood T.K., Flower D.R.  Alpha Helix Transmembrane Proteins: Enhanced prediction using a Bayesian approach.  Bioinformation 2006 1: 234-236.

Davies, M.N., Toseland, C.P., Moss, D.S. and Flower, D.R. Benchmarking pKa Prediction. BMC Biochemistry 2006 7:18. (Joint First Authors)

Hattotuwagama, C.K., Toseland, C.P., Guan, P., Taylor, D.J., Hemsley, S.L., Doytchinova, I.A. and Flower, D.R. Toward Prediction of Class II Mouse Major Histocompatibility Complex Peptide Binding Affinity: in Silico Bioinformatic Evaluation Using Partial Least Squares, a Robust Multivariate Statistical Technique. J. Chem. Inf. Model. 2006 46:1491-1502.

Toseland, C.P., McSparron, H. and Flower, D.R. “PPD v1.0—an integrated, web-accessible database of experimentally determined pKa values.” Nucleic Acid Research 2006 34:D199-203.


Guan, P., Davies, M., Taylor, D.J., Wan, S., McSparron, H., Hemsley, S.L., Toseland, C., Blythe, M.J., Taylor, P.D., Walshe, V., Hattotuwagama, C.K., Doytchinova, I.A., Coveney, P.V., Borrow, P. and Flower, D.R. “Computational Chemistry, Informatics, and the Discovery of Vaccines.” Current Computer-Aided Drug Design 2005 1:377-398.

Toseland, C.P., McSparron, H. and Flower, D.R. “DSD - An integrated, web-accessible database of Dehydrogenase Enzyme Stereospecificities.” BMC Bioinformatics 2005 6:283. 

Toseland, C.P., Clayton, D.J., McSparron, H., Hemsley, S.L., Blythe, M.J., Paine, K., Doytchinova, I.A., Guan, P., Hattotuwagama, C.K. and Flower, D.R. “AntiJen: a quantitative immunology database integrating functional, thermodynamic, kinetic, biophysical, and cellular data.” Immunome Reseach 2005, 1:4.

bottom of page